Difference between revisions of "Part:BBa K4765021"
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− | The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA. | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> |
+ | __TOC__ | ||
+ | |||
+ | ===Introduction=== | ||
+ | The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.<ref> Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ''ACS Synthetic Biology, 12''(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref>. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of ''E. coli'' for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA. | ||
+ | |||
+ | ===Characterization=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
− | <partinfo> | + | <partinfo>BBa_K4765021 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K4765021 parameters</partinfo> |
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+ | ==References== | ||
+ | <references /> |
Latest revision as of 12:23, 12 October 2023
stem-loop 1, from 10.1021/acssynbio.2c00416
Introduction
The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.[1].
Usage and Biology
In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of E. coli for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416