Difference between revisions of "Part:BBa K4656002"
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<partinfo>BBa_K4656002 short</partinfo>
 
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TPH also plays a crucial role in the synthesis of serotonin, where the rate-limiting step is catalyzed by TPH to convert tryptophan (an essential amino acid) to 5-hydroxytryptophan (5-HTP).
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<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;cursor: zoom-in;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/tdc-tph.jpg" width="600" height="200"></center></html>
  
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Tph1 is the gene encoding tryptophan hydroxylase (TPH), which plays an important role in the synthesis of serotonin (5-hydroxytryptamine, 5-HT), in which the rate-limiting step is catalyzed by TPH. The Tph1 gene expresses the TPH1 protein, which converts tryptophan (an essential amino acid) to 5-hydroxytryptophan (5-HTP).
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<center><b>The synthesis of 5-HT </b></center>
  
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As a neurotransmitter, serotonin can play the role of intestinal motility. If its secretion is insufficient, it can easily lead to the decline of intestinal motility, and then lead to chronic constipation. Therefore, constructing plasmids introducing Tph1 gene and promoting TPH expression is conducive to the treatment of chronic constipation.
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===Usage and Biology===
 
===Usage and Biology===
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As a neurotransmitter, serotonin plays a role in intestinal motility. If secretion in the gut is insufficient, it can easily lead to the decline of intestinal motility, and then lead to chronic constipation. Therefore, promoting TPH expression is conducive to the treatment of chronic constipation. Experimental results about our 5-HT producing circuit can be found in our composite part BBa K4656007.
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<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;cursor: zoom-in;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/part-plam-tph-tdc.jpg" width="600" height="300"></center></html>
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4656002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4656002 SequenceAndFeatures</partinfo>
  

Latest revision as of 12:42, 5 October 2023


TPH

TPH also plays a crucial role in the synthesis of serotonin, where the rate-limiting step is catalyzed by TPH to convert tryptophan (an essential amino acid) to 5-hydroxytryptophan (5-HTP).

The synthesis of 5-HT


Usage and Biology

As a neurotransmitter, serotonin plays a role in intestinal motility. If secretion in the gut is insufficient, it can easily lead to the decline of intestinal motility, and then lead to chronic constipation. Therefore, promoting TPH expression is conducive to the treatment of chronic constipation. Experimental results about our 5-HT producing circuit can be found in our composite part BBa K4656007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 898
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 240