Difference between revisions of "Part:BBa K4883002"
(One intermediate revision by the same user not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K4883002 short</partinfo> | <partinfo>BBa_K4883002 short</partinfo> | ||
− | + | RIB7 encodes for diaminohydroxyphoshoribosylaminopyrimidine deaminase in S. cerevisiae. This enzyme catalyzes the second step of the riboflavin biosynthesis pathway, transforming 2,5-diamino-6-(5-phospho-D-ribosylamino)-pyrimidin-4(3H)-one (DARPP) to 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5 -phosphate (DArPP) (Gudipati et al., 2014). | |
− | |||
− | |||
<!-- --> | <!-- --> | ||
Line 17: | Line 15: | ||
<partinfo>BBa_K4883002 parameters</partinfo> | <partinfo>BBa_K4883002 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here--> | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | RIB7 is a riboflavin synthase. Overexpression of RIB7 could lead to overproduction of vitamin B2 in S. cerevisiae. | ||
+ | |||
+ | <html> | ||
+ | <body> | ||
+ | <figure> | ||
+ | <div class = "center"> | ||
+ | <center><img src = "https://static.igem.wiki/teams/4883/wiki/part-bba-k4883002-1.png" style = "width:480px"></center> | ||
+ | </div> | ||
+ | <figcaption><center>Figure 1. Gel electrophoresis of colony PCR products for verification of correct transformation of plasmid pCEV-G4-RIB7-Km into E. coli DH5α. </center></figcaption> | ||
+ | </figure> | ||
+ | </body> | ||
+ | </html> | ||
+ | |||
+ | ==References== | ||
+ | Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015. |
Latest revision as of 01:47, 6 October 2023
RIB7, GTP cyclohydrolase II from Saccharomyces cerevisiae
RIB7 encodes for diaminohydroxyphoshoribosylaminopyrimidine deaminase in S. cerevisiae. This enzyme catalyzes the second step of the riboflavin biosynthesis pathway, transforming 2,5-diamino-6-(5-phospho-D-ribosylamino)-pyrimidin-4(3H)-one (DARPP) to 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5 -phosphate (DArPP) (Gudipati et al., 2014).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 240
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 172
Usage and Biology
RIB7 is a riboflavin synthase. Overexpression of RIB7 could lead to overproduction of vitamin B2 in S. cerevisiae.
References
Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015.