Difference between revisions of "Part:BBa K4882003"
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<partinfo>BBa_K4882003 short</partinfo> | <partinfo>BBa_K4882003 short</partinfo> | ||
− | + | This is a red fluorescent protein SuperNova fused with an SV40 nuclear localization signal (NLS). | |
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<partinfo>BBa_K4882003 parameters</partinfo> | <partinfo>BBa_K4882003 parameters</partinfo> | ||
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+ | <!-- Add more about the biology of this part here--> | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | Metarhizium anisopliae is an entomopathogenic fungus widely used as a biopesticide. SuperNova ([https://parts.igem.org/Part:BBa_K4882008 BBa_K4882008]) is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of green-yellow light (~579 nm). To improve the safety of M. anisopliae, SuperNova is introduced as a part of a suicide switch. The SV40 NLS ([https://parts.igem.org/Part:BBa_K2549054 BBa_K2549054]) should guide the protein to be transported into the cell nucleus, and let ROS attack the most vulnerable genomic DNA (Paardekooper et al., 2019). | ||
+ | |||
+ | ==References== | ||
+ | Paardekooper LM, van Vroonhoven E, Ter Beest M, van den Bogaart G. Radical Stress Is More Cytotoxic in the Nucleus than in Other Organelles. Int J Mol Sci. 2019 Aug 25;20(17):4147. doi: 10.3390/ijms20174147. PMID: 31450682; PMCID: PMC6747261. |
Latest revision as of 14:35, 4 October 2023
SuperNova-SV40 NLS
This is a red fluorescent protein SuperNova fused with an SV40 nuclear localization signal (NLS).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 714
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 151
Illegal BsaI.rc site found at 442
Usage and Biology
Metarhizium anisopliae is an entomopathogenic fungus widely used as a biopesticide. SuperNova (BBa_K4882008) is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of green-yellow light (~579 nm). To improve the safety of M. anisopliae, SuperNova is introduced as a part of a suicide switch. The SV40 NLS (BBa_K2549054) should guide the protein to be transported into the cell nucleus, and let ROS attack the most vulnerable genomic DNA (Paardekooper et al., 2019).
References
Paardekooper LM, van Vroonhoven E, Ter Beest M, van den Bogaart G. Radical Stress Is More Cytotoxic in the Nucleus than in Other Organelles. Int J Mol Sci. 2019 Aug 25;20(17):4147. doi: 10.3390/ijms20174147. PMID: 31450682; PMCID: PMC6747261.