Difference between revisions of "Part:BBa K185005"

 
 
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<partinfo>BBa_K185005 short</partinfo>
 
<partinfo>BBa_K185005 short</partinfo>
  
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On this vector, RelB-antitoxin is under control of L-Trytophan(Lamda cI Promoter), while RelE-toxin is regulated by IPTG(Lac Promoter). We use this part to check the function of TA(toxin-antitoxin system).Compared with nagative control, when IPTG is provided, the genetically modified E.coli turns to dormancy. When IPTG and Tryptophan are both provided, the  E.coli grows normally, suggesting that RelB works as an anti-toxin factor to rescue dormant bacteria.
  
 
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Latest revision as of 07:20, 19 October 2009

Tryptophan-Induced RelB-antitoxin&Arabinose-Induced RelE-toxin

On this vector, RelB-antitoxin is under control of L-Trytophan(Lamda cI Promoter), while RelE-toxin is regulated by IPTG(Lac Promoter). We use this part to check the function of TA(toxin-antitoxin system).Compared with nagative control, when IPTG is provided, the genetically modified E.coli turns to dormancy. When IPTG and Tryptophan are both provided, the E.coli grows normally, suggesting that RelB works as an anti-toxin factor to rescue dormant bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2696
    Illegal XhoI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2513