Difference between revisions of "Part:BBa K4297040"

Latest revision as of 03:22, 14 October 2022

pYLXP'-ADH7

The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase ADH7, which can catalyze the reaction of phenylacetaldehyde to 2-PE.The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 394.50 mg/L.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1293
Illegal NheI site found at 5557
Illegal NotI site found at 1448
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 249
Illegal XhoI site found at 4316
Illegal XhoI site found at 4349
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4910
Illegal AgeI site found at 950
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI site found at 1284
Illegal BsaI site found at 4596
Illegal BsaI site found at 4997
Illegal BsaI.rc site found at 255
Illegal BsaI.rc site found at 4582
Illegal BsaI.rc site found at 5653

Revision as of 03:21, 14 October 2022 (view source) GYang1213 (Talk \| contribs) ← Older edit		Latest revision as of 03:22, 14 October 2022 (view source) GYang1213 (Talk \| contribs)
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	<partinfo>BBa_K4297040 short</partinfo>		<partinfo>BBa_K4297040 short</partinfo>

−	The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase ~~ADH8~~, which can catalyze the reaction of phenylacetaldehyde to 2-PE.The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of ~~507~~.33 mg/L.	+	The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase ADH7, which can catalyze the reaction of phenylacetaldehyde to 2-PE.The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 394.50 mg/L.