Difference between revisions of "Part:BBa K4297049"
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| − | The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. | + | The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 501.10 mg/L. |
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Latest revision as of 03:15, 14 October 2022
PYLXP'-PAR8
The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 501.10 mg/L.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1293
Illegal NotI site found at 1448 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 249
Illegal BamHI site found at 5109
Illegal BamHI site found at 5554
Illegal XhoI site found at 4316
Illegal XhoI site found at 4349 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 950
Illegal AgeI site found at 4585 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 1284
Illegal BsaI site found at 4588
Illegal BsaI.rc site found at 255
Illegal BsaI.rc site found at 4582
Illegal BsaI.rc site found at 5618