Difference between revisions of "Part:BBa K4297045"

Latest revision as of 03:12, 14 October 2022

PYLXP'-PAR4

The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR4, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 786.58 mg/L.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1293
Illegal NotI site found at 1448
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 249
Illegal BamHI site found at 4859
Illegal BamHI site found at 4880
Illegal XhoI site found at 4316
Illegal XhoI site found at 4349
Illegal XhoI site found at 5586
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 950
Illegal AgeI site found at 4585
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI site found at 1284
Illegal BsaI site found at 4588
Illegal BsaI.rc site found at 255
Illegal BsaI.rc site found at 4582
Illegal BsaI.rc site found at 5010
Illegal BsaI.rc site found at 5600

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4297045 short</partinfo>
-The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR4, which can catalyze the reaction of phenylacetaldehyde to 2-PE.
+The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR4, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 786.58 mg/L.
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