Difference between revisions of "Part:BBa K173010:Experience"
(→Materials) |
|||
| (One intermediate revision by one other user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K173010=== | ===Applications of BBa_K173010=== | ||
| + | |||
| + | This part was not working in a preliminary induction test. | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | ===='''Materials'''==== | ||
| + | *Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20% | ||
| + | *Antibiotics were: Ampicillin (Amp) 100 ug/ml. All of them were stored at -20°C in 1000x stocks. | ||
| + | *LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH. | ||
| + | *Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 13:53, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K173010
This part was not working in a preliminary induction test.
Materials
- Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20%
- Antibiotics were: Ampicillin (Amp) 100 ug/ml. All of them were stored at -20°C in 1000x stocks.
- LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH.
- Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock.
User Reviews
UNIQ5bcf5cd67d036e46-partinfo-00000000-QINU UNIQ5bcf5cd67d036e46-partinfo-00000001-QINU