Difference between revisions of "Part:BBa K203106:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | * The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA): Plasmid 14947, pHMGCS-Luc (aka: pES8) | ||
+ | * We used the following primers with BBb_standard for the amplification and isolation of the HMG CoA synthase promoter: | ||
+ | ** primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTCGATCTCCTCTCACTGACCTTC-3' | ||
+ | ** primer reverse: 5'-GATCCTGCAGGCGGCCGCGCTAGCGCAGCTTTATAGCCACCGACCC-3' | ||
===Source=== | ===Source=== | ||
− | + | Addgene, Cambridge (USA): Plasmid 14947: pHMGCS-Luc (aka: pES8). | |
===References=== | ===References=== | ||
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+ | Castoreno, A. B. et al. Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Proc Natl Acad Sci U S A 102, 13129–13134 (2005). |
Latest revision as of 10:42, 19 October 2009
HMG-CoA synthase promoter (sterol regulated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA): Plasmid 14947, pHMGCS-Luc (aka: pES8)
- We used the following primers with BBb_standard for the amplification and isolation of the HMG CoA synthase promoter:
- primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTCGATCTCCTCTCACTGACCTTC-3'
- primer reverse: 5'-GATCCTGCAGGCGGCCGCGCTAGCGCAGCTTTATAGCCACCGACCC-3'
Source
Addgene, Cambridge (USA): Plasmid 14947: pHMGCS-Luc (aka: pES8).
References
Castoreno, A. B. et al. Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Proc Natl Acad Sci U S A 102, 13129–13134 (2005).