Difference between revisions of "Part:BBa K200028:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | The clone for PAH was obtained from Imagenes (Library : 1643 (Human Liver cDNA), Set : 969 (IRAU MGC Human verified full length cam cDNA), Source: liver))<br> | ||
Site directed mutagenesis was carried out to obtain the desired characteristics | Site directed mutagenesis was carried out to obtain the desired characteristics | ||
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===Source=== | ===Source=== | ||
Latest revision as of 15:33, 21 October 2009
Protease resistant PAH
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 287
Illegal BamHI site found at 814
Illegal XhoI site found at 524 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The clone for PAH was obtained from Imagenes (Library : 1643 (Human Liver cDNA), Set : 969 (IRAU MGC Human verified full length cam cDNA), Source: liver))
Site directed mutagenesis was carried out to obtain the desired characteristics
Source
PAH from Homo sapiens.