Difference between revisions of "Part:BBa K4444018:Design"

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===Design Notes===
 
===Design Notes===
  
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<u>Modifications to FRB-cTEV:
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<u>Modifications to FKBP-cTEV:</u> (<html><ref>1</ref></html>)
  
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</u>- FRB is replaced with sspB-BFP
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- FKBP is replaced with sspB-BFP
  
  
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<u>Components:
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<u>Components:</u>
  
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</u>sspB, BFP, cTEV
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    <figure data-ref="1">
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        <img class="cTEVsspb" src="https://static.igem.wiki/teams/4444/wiki/parts-pictures/ctevsspb.png" width="500">
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        <figcaption>The cTEV-sspB System contains sspB, BFP, cTEV
  
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<html><img src="https://static.igem.wiki/teams/4444/wiki/parts-pictures/ctevsspb.png" width="500px"></html>
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        </figcaption>
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    </figure>
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===Source===
 
===Source===

Latest revision as of 16:13, 13 October 2022

Design Notes

Modifications to FKBP-cTEV: (1)

- FKBP is replaced with sspB-BFP


Components:

The cTEV-sspB System contains sspB, BFP, cTEV

Source

FRB-cTEV: https://www.addgene.org/179676/

sspB and BFP: Lab member’s plasmid (William Benman) – pHR sspB(nano)-BFP-SOS-2A-iLID

References

[1] Wehr, M., Laage, R., Bolz, U. et al. Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3, 985–993 (2006). https://doi.org/10.1038/nmeth967

[2] Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.