Difference between revisions of "Part:BBa K4444018:Design"
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===Design Notes===
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<html lang="en">
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<head>
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    <meta charset="UTF-8">
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    <title></title>
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    <style>
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        .ui.medium.image {
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            width: 100%;
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        }
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    </style>
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    <script type="text/javascript">
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        $(this).find('ref').each(function () {
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            var label = $(this).text();
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            $(this).click(function (event) {
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                event.preventDefault();
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            var number = figures[1][label];
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            $(this).html("<a href=''>Figure " + figures[1][label] + "</a>");
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                window.scroll(0, $(figures[0][number]).offset().top + 100);
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                    console.log(refName, "This label has already been assigned.");
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                    $(figures[0][number]).children('figcaption').prepend("Figure " + number + ": ")
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                var number = figures[1][label];
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                $(this).html("<a href=''>Figure " + figures[1][label] + "</a>");
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</head>
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<body>
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</html>
  
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<u>Modifications to FRB-cTEV:
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===Design Notes===
  
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</u>- FRB is replaced with sspB-BFP
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<u>Modifications to FKBP-cTEV:</u> (<html><ref>1</ref></html>)
  
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- FKBP is replaced with sspB-BFP
  
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<u>Components:
 
  
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</u>sspB, BFP, cTEV
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<u>Components:</u>
  
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<html><img src="https://drive.google.com/file/d/1f4QzNCvjes7BjYfngbbrQYFPGCnudgJe/view?usp=sharing" width="500px"></html>
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<html>
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<div>
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    <figure data-ref="1">
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        <img class="cTEVsspb" src="https://static.igem.wiki/teams/4444/wiki/parts-pictures/ctevsspb.png" width="500">
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        <figcaption>The cTEV-sspB System contains sspB, BFP, cTEV
  
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        </figcaption>
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    </figure>
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</div>
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</html>
  
 
===Source===
 
===Source===

Latest revision as of 16:13, 13 October 2022

Design Notes

Modifications to FKBP-cTEV: (1)

- FKBP is replaced with sspB-BFP


Components:

The cTEV-sspB System contains sspB, BFP, cTEV

Source

FRB-cTEV: https://www.addgene.org/179676/

sspB and BFP: Lab member’s plasmid (William Benman) – pHR sspB(nano)-BFP-SOS-2A-iLID

References

[1] Wehr, M., Laage, R., Bolz, U. et al. Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3, 985–993 (2006). https://doi.org/10.1038/nmeth967

[2] Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.