Difference between revisions of "Part:BBa K4307026"

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This part is the transcription factor of Affi-PmrB/A system（BBa_K4307028）, which is modified from the PmrB/A two-component system in <i>E.coli</i>. It is phosphorylated by AffipmrB, then it can activate PmrC promoter.
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This part is the transcription factor of Affi-PmrB/A system（BBa_K4307042）, which is modified from the PmrB/A two-component system in <i>E.coli</i>. It is phosphorylated by AffipmrB, then it can activate PmrC promoter.
  
  
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<span class='h3bb'>Sequence and Features</span>
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<h2>Sequence and Features</h2>
 
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<partinfo>BBa_K4307026 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K4307000 parameters</partinfo>
 
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<h2>Characterization </h2>
 
<h2>Characterization </h2>
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https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307026-gel.png<br>
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                 <b>Figure 1: </b> <b>The construction results of PmrA.</b>
 
                 <b>Figure 1: </b> <b>The construction results of PmrA.</b>
 
                  
 
                  
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Latest revision as of 16:53, 13 October 2022


PmrA

This part is the transcription factor of Affi-PmrB/A system（BBa_K4307042）, which is modified from the PmrB/A two-component system in E.coli. It is phosphorylated by AffipmrB, then it can activate PmrC promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


Figure 1: The construction results of PmrA.