Difference between revisions of "Part:BBa K4390066"
 
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<partinfo>BBa_K4390066 short</partinfo>
 
<partinfo>BBa_K4390066 short</partinfo>
  
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This is an O part consisting of two SpyCatcher domains separated by a glycine-serine linker. The SpyCatcher domains are used for binding and crosslinking proteins that have a SpyTag attached to them to create a mesh that can spontaneously form a hydrogel. The glycine-serine linker forms an unstructured domain that allows for greater flexibility of the two SpyCatcher domains, while minimising the risk of the two domains hindering each other's functions. The 2022 Edinburgh-UHAS iGEM team used an expressed level 1 construct containing this part to crosslink metallothioneins tagged with a SpyTag domain.
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
  
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<!-- Add more about the biology of this part here
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'''This is a Level 0 part of type O part generating the following 4 base overhangs at upstream (AGCC) and downstream (TTCG) ends.'''
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===Usage and Biology===
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==Usage and Biology==
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This is an O part consisting of two SpyCatcher domains separated by a glycine-serine linker. The SpyCatcher domains are used for binding and crosslinking proteins that have a SpyTag attached to them to create a mesh that can spontaneously form a hydrogel. The glycine-serine linker forms an unstructured domain that allows for greater flexibility of the two SpyCatcher domains, while minimising the risk of the two domains hindering each other's functions. The 2022 Edinburgh-UHAS iGEM team used an expressed level 1 construct containing this part to crosslink metallothioneins tagged with a SpyTag domain.
  
 
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<span class='h3bb'>Sequence and Features</span>
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==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K4390066 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4390066 SequenceAndFeatures</partinfo>
  

Latest revision as of 23:55, 13 October 2022


SpyCatcher O part

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is a Level 0 part of type O part generating the following 4 base overhangs at upstream (AGCC) and downstream (TTCG) ends.

Usage and Biology

This is an O part consisting of two SpyCatcher domains separated by a glycine-serine linker. The SpyCatcher domains are used for binding and crosslinking proteins that have a SpyTag attached to them to create a mesh that can spontaneously form a hydrogel. The glycine-serine linker forms an unstructured domain that allows for greater flexibility of the two SpyCatcher domains, while minimising the risk of the two domains hindering each other's functions. The 2022 Edinburgh-UHAS iGEM team used an expressed level 1 construct containing this part to crosslink metallothioneins tagged with a SpyTag domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]