Difference between revisions of "Part:BBa K4307009"

m
m
 
(2 intermediate revisions by one other user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K43070009 short</partinfo>
+
<partinfo>BBa_K4307009 short</partinfo>
  
 
Originated from <i>Lactococcus lactis subsp. Lactis</i>, PnisA is a member of the two-component regulatory system NisK/NisR involved in the regulation of the biosynthesis of lantibiotic nisin. PnisA functions as a promoter which is activated by phosphorylated NisR and enhance downstream gene expression in response to environmental nisin signals. We managed to involve PnisA sequence in plasmids for <i>E.coli</i> strain.<br>
 
Originated from <i>Lactococcus lactis subsp. Lactis</i>, PnisA is a member of the two-component regulatory system NisK/NisR involved in the regulation of the biosynthesis of lantibiotic nisin. PnisA functions as a promoter which is activated by phosphorylated NisR and enhance downstream gene expression in response to environmental nisin signals. We managed to involve PnisA sequence in plasmids for <i>E.coli</i> strain.<br>
−
Functions together with NisK(BBa_K4307007), NisR(BBa_K4307008), J23100 and EGFP, it forms composite part J23100-nisK-nisR+PnisA-EGFP(BBa_K4307013) to express fluorescence signal induced by nisin.
+
Functioned together with NisK(BBa_K4307007), NisR(BBa_K4307008), J23100 and EGFP, it forms composite part J23100-nisK-nisR+PnisA-EGFP(BBa_K4307045) to express fluorescence signal induced by nisin.
  
  
Line 12: Line 12:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<h2>Sequence and Features</h2>
−
<partinfo>BBa_K43070009 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K4307009 SequenceAndFeatures</partinfo>
  
  
Line 20: Line 20:
 
<partinfo>BBa_K4307000 parameters</partinfo>
 
<partinfo>BBa_K4307000 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<html>
  
 
<h2>Characterization</h2>
 
<h2>Characterization</h2>
Line 26: Line 28:
  
 
<br>
 
<br>
−
https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307009-gel.png<br>
+
<div class="center">
 +
    <div class="thumb tnone">
 +
        <div class="thumbinner" style="width:50%;">
 +
            <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307009-gel.png" class="image">
 +
                <img alt="" src="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307009-gel.png" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
 +
                <div class="magnify">
 +
                    <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307009-gel.png" class="internal" title="Enlarge"></a>
 +
                </div>
 
                 <b>Figure 1: </b> <b>The construction results of PnisA.</b>
 
                 <b>Figure 1: </b> <b>The construction results of PnisA.</b>
 
                  
 
                  
−
         
+
            </div>
 +
        </div>
 +
    </div>
 +
</div>
 +
 
 +
</html>
  
  

Latest revision as of 16:30, 13 October 2022


PnisA

Originated from Lactococcus lactis subsp. Lactis, PnisA is a member of the two-component regulatory system NisK/NisR involved in the regulation of the biosynthesis of lantibiotic nisin. PnisA functions as a promoter which is activated by phosphorylated NisR and enhance downstream gene expression in response to environmental nisin signals. We managed to involve PnisA sequence in plasmids for E.coli strain.
Functioned together with NisK(BBa_K4307007), NisR(BBa_K4307008), J23100 and EGFP, it forms composite part J23100-nisK-nisR+PnisA-EGFP(BBa_K4307045) to express fluorescence signal induced by nisin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


Figure 1: The construction results of PnisA.