Difference between revisions of "Part:BBa K4390025"
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<partinfo>BBa_K4390025 short</partinfo> | <partinfo>BBa_K4390025 short</partinfo> | ||
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]''' | '''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]''' | ||
| + | '''This is a Level 0 part of type N-O part generating the following 4 base overhangs at upstream (AATG).''' | ||
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| + | ==Usage and Biology== | ||
This is an NO part containing a silica-binding tag and a glycine-serine linker. The silica-binding tag allows for the expressed level 1 construct to bind to silica, while the glycine-serine linker introduces a short unstructured domain, minimising the risk of the silica tag interfering with the activity of other parts. The 2022 Edinburgh-UHAS iGEM team used this part to bind metallothioneins to silica beads. It does not have TTCG at the end to complete the sequence, as this is regenerated by the O part 3' fusion site in JUMP assembly. Add TTCG at the end to use as a normal part. | This is an NO part containing a silica-binding tag and a glycine-serine linker. The silica-binding tag allows for the expressed level 1 construct to bind to silica, while the glycine-serine linker introduces a short unstructured domain, minimising the risk of the silica tag interfering with the activity of other parts. The 2022 Edinburgh-UHAS iGEM team used this part to bind metallothioneins to silica beads. It does not have TTCG at the end to complete the sequence, as this is regenerated by the O part 3' fusion site in JUMP assembly. Add TTCG at the end to use as a normal part. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | ==<span class='h3bb'>Sequence and Features</span>== |
<partinfo>BBa_K4390025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4390025 SequenceAndFeatures</partinfo> | ||
Latest revision as of 23:25, 13 October 2022
Si-tag :: Gly-Ser Linker
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
This is a Level 0 part of type N-O part generating the following 4 base overhangs at upstream (AATG).
Usage and Biology
This is an NO part containing a silica-binding tag and a glycine-serine linker. The silica-binding tag allows for the expressed level 1 construct to bind to silica, while the glycine-serine linker introduces a short unstructured domain, minimising the risk of the silica tag interfering with the activity of other parts. The 2022 Edinburgh-UHAS iGEM team used this part to bind metallothioneins to silica beads. It does not have TTCG at the end to complete the sequence, as this is regenerated by the O part 3' fusion site in JUMP assembly. Add TTCG at the end to use as a normal part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 14
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]