Difference between revisions of "Part:BBa K4438904"
 
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<partinfo>BBa_K4438904 short</partinfo>
 
<partinfo>BBa_K4438904 short</partinfo>
  

Latest revision as of 15:06, 12 October 2022

crp_Target_2

crp_Target_2 (BBa_K4438904) is a single-stranded DNA having 83 nucleotides. The 5’ end has few bases complementary to crp_trigger_2_phi29(BBa_K4438903), the middle region has ssDNA sense T7 promoter sequence and the 5’ end has ssDNA sense broccoli sequence.

Usage and Biology

This part along with crp_trigger_2_phi29(BBa_K4438903) and Aptamer_crp (BBa_K4438900) can be used to detect different concentrations of CRP [1].

T--IISER-Tirupati India--hsa-crp-2.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.