Difference between revisions of "Part:BBa K4438905"

 
 
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__NOTOC__
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<partinfo>BBa_K4438903 short</partinfo>
  
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crp_trigger_3_phi29 (<partinfo>BBa_K4438905</partinfo>) is a single-stranded DNA having 34 nucleotides. Figure 1A) shows the secondary structure and minimum free energy. Its 3’ end has a few bases complementary to the part Aptamer_crp (BBa_K4438900). At the 5’ end, there are few bases of broccoli cDNA. The middle region has an ssDNA antisense T7 promoter sequence.
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===Usage and Biology===
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The part crp_trigger_3_phi29 (<partinfo>BBa_K4438905</partinfo>) binds to the part Aptamer_crp (<partinfo>BBa_K4438900</partinfo>) due to complementarity. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. CRP binds with Aptamer_crp (<partinfo>BBa_K4438900</partinfo>) and displaces the crp_trigger_3_phi29 (<partinfo>BBa_K4438905</partinfo>) [1]. This part has complete complementarity with part crp_Target_3(<partinfo>BBa_K4438906</partinfo>). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
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Different levels of CRP can be detected using all these three parts.
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[[File:T--IISER-Tirupati_India--hsa-crp-3.png]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4438905 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4438905 parameters</partinfo>
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===References===
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Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.

Latest revision as of 14:07, 12 October 2022

crp_trigger_2_phi29

crp_trigger_3_phi29 (BBa_K4438905) is a single-stranded DNA having 34 nucleotides. Figure 1A) shows the secondary structure and minimum free energy. Its 3’ end has a few bases complementary to the part Aptamer_crp (BBa_K4438900). At the 5’ end, there are few bases of broccoli cDNA. The middle region has an ssDNA antisense T7 promoter sequence.


Usage and Biology

The part crp_trigger_3_phi29 (BBa_K4438905) binds to the part Aptamer_crp (BBa_K4438900) due to complementarity. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. CRP binds with Aptamer_crp (BBa_K4438900) and displaces the crp_trigger_3_phi29 (BBa_K4438905) [1]. This part has complete complementarity with part crp_Target_3(BBa_K4438906). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of CRP can be detected using all these three parts.

T--IISER-Tirupati India--hsa-crp-3.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.