Difference between revisions of "Part:BBa K4439003"
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==Abstract== | ==Abstract== | ||
| − | + | Avitag is an unique 15 amino acid peptide tag using the biotin ligase (BirA) from E. coli. It allows highly targeted enzymatic conjugation for biotinylation. Avitag creates an ideal presentation for molecular binding interactions. For the scope of HESTIA's project, we used Avitag to allow a strong binding between a recombinant silk protein ([[Part:BBa K4439007]]) forming a hydrophobic biofilm and a potential fire-retardancy protein ([[Part:BBa K4439015]]) constituting a test for a modular protein coating. | |
==Sequence and Features== | ==Sequence and Features== | ||
<partinfo>BBa_K4439003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4439003 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | ==References== | ||
| + | |||
| + | <ul> | ||
| + | <li> Welcome to Avidity. (s. d.). Visited october 10th 2022, at https://www.avidity.com/ | ||
| + | |||
| + | </li> | ||
| + | </ul> | ||
Latest revision as of 14:20, 12 October 2022
Avitag
Abstract
Avitag is an unique 15 amino acid peptide tag using the biotin ligase (BirA) from E. coli. It allows highly targeted enzymatic conjugation for biotinylation. Avitag creates an ideal presentation for molecular binding interactions. For the scope of HESTIA's project, we used Avitag to allow a strong binding between a recombinant silk protein (Part:BBa K4439007) forming a hydrophobic biofilm and a potential fire-retardancy protein (Part:BBa K4439015) constituting a test for a modular protein coating.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Welcome to Avidity. (s. d.). Visited october 10th 2022, at https://www.avidity.com/