Difference between revisions of "Part:BBa K4361113"
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<partinfo>BBa_K4361113 short</partinfo> | <partinfo>BBa_K4361113 short</partinfo> | ||
− | A site-directed mutagenesis primer, to be used in conjunction with the mutation-introducingforward primer [[Part:BBa_K4361112]]. Primers can be used in a PCR reaction with the | + | A site-directed mutagenesis primer, to be used in conjunction with the mutation-introducingforward primer [[Part:BBa_K4361112]]. Primers can be used in a PCR reaction with the [[Part:BBa_K4361115]] part as DNA template. A deletion will be introduced in the <i>blc</i> operator sequence, resulting in the altered sequence as described in [[Part:BBa_K4361114]]. |
Latest revision as of 13:42, 12 October 2022
Blc operator SDM primer R
A site-directed mutagenesis primer, to be used in conjunction with the mutation-introducingforward primer Part:BBa_K4361112. Primers can be used in a PCR reaction with the Part:BBa_K4361115 part as DNA template. A deletion will be introduced in the blc operator sequence, resulting in the altered sequence as described in Part:BBa_K4361114.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 21
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 21
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 21
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 21
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 21
- 1000COMPATIBLE WITH RFC[1000]