Difference between revisions of "Part:BBa K4509769:Design"

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===References===
 
===References===
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1)[https://www.sciencedirect.com/science/article/abs/pii/0022283686901713 Freudl, R., MacIntyre, S., Degen, M., & Henning, U. (1986). Cell surface exposure of the outer membrane protein OmpA of Escherichia coli K-12. Journal of molecular biology, 188(3), 491-494.]
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2)[https://journals.asm.org/doi/full/10.1128/IAI.00096-09 Gaddy, J. A., Tomaras, A. P., & Actis, L. A. (2009). The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infection and immunity, 77(8), 3150-3160.]
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3)[https://onlinelibrary.wiley.com/doi/abs/10.1002/elsc.201700200 3.Kleiner‐Grote, G. R., Risse, J. M., & Friehs, K. (2018). Secretion of recombinant proteins from E. coli. Engineering in Life Sciences, 18(8), 532-550.]
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4)[https://www.jbc.org/article/S0021-9258(18)45908-X/fulltext Sugawara, E., & Nikaido, H. (1992). Pore-forming activity of OmpA protein of Escherichia coli. Journal of Biological Chemistry, 267(4), 2507-2511.]
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5)[https://www.sciencedirect.com/science/article/abs/pii/S0006291X0296657X 5.Wang, Y. (2002). The function of OmpA in Escherichia coli. Biochemical and biophysical research communications, 292(2), 396-401.]

Latest revision as of 12:21, 12 October 2022


Cell surface aphA with constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 966


Design Notes

The constitutive promoter BBa_J23100 has been used since the strength of BBa_J23100 is more. This will facilitate overexpression of the protein on the cell surface.


References

1)Freudl, R., MacIntyre, S., Degen, M., & Henning, U. (1986). Cell surface exposure of the outer membrane protein OmpA of Escherichia coli K-12. Journal of molecular biology, 188(3), 491-494.

2)Gaddy, J. A., Tomaras, A. P., & Actis, L. A. (2009). The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infection and immunity, 77(8), 3150-3160.

3)3.Kleiner‐Grote, G. R., Risse, J. M., & Friehs, K. (2018). Secretion of recombinant proteins from E. coli. Engineering in Life Sciences, 18(8), 532-550.

4)Sugawara, E., & Nikaido, H. (1992). Pore-forming activity of OmpA protein of Escherichia coli. Journal of Biological Chemistry, 267(4), 2507-2511.

5)5.Wang, Y. (2002). The function of OmpA in Escherichia coli. Biochemical and biophysical research communications, 292(2), 396-401.