Difference between revisions of "Part:BBa K4501022"
 
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Disclaimer. This is a plasmid backbone and therefore the compatibility labels are not applicable.
 
Disclaimer. This is a plasmid backbone and therefore the compatibility labels are not applicable.
 
This plasmid backbone, based on the pJUMP29-1A KanR TypeIIS Level 1 pUC backbone from the distribution kit, has been upgraded to incorporate the 5' and 3' modules required for a lentiviral transfer plasmid of 2nd and 3rd generation. The modules (BBa_K4501005 and BBa_K4501006) are incorporated on the secondary restriction sites for BbsI and PaqCI located at the sides of the main assembly site. The plasmid is ready for a BsmbI-based cloning assembly and subsequent direct use as transfer plasmid.
 
This plasmid backbone, based on the pJUMP29-1A KanR TypeIIS Level 1 pUC backbone from the distribution kit, has been upgraded to incorporate the 5' and 3' modules required for a lentiviral transfer plasmid of 2nd and 3rd generation. The modules (BBa_K4501005 and BBa_K4501006) are incorporated on the secondary restriction sites for BbsI and PaqCI located at the sides of the main assembly site. The plasmid is ready for a BsmbI-based cloning assembly and subsequent direct use as transfer plasmid.
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[[File:pJUMP.jpg]]
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Figure 1. Map sequence of the pJUMP-based lentiviral transfer plasmid.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:22, 12 October 2022


pJUMP lentiviral transfer plasmid

Disclaimer. This is a plasmid backbone and therefore the compatibility labels are not applicable. This plasmid backbone, based on the pJUMP29-1A KanR TypeIIS Level 1 pUC backbone from the distribution kit, has been upgraded to incorporate the 5' and 3' modules required for a lentiviral transfer plasmid of 2nd and 3rd generation. The modules (BBa_K4501005 and BBa_K4501006) are incorporated on the secondary restriction sites for BbsI and PaqCI located at the sides of the main assembly site. The plasmid is ready for a BsmbI-based cloning assembly and subsequent direct use as transfer plasmid.

PJUMP.jpg Figure 1. Map sequence of the pJUMP-based lentiviral transfer plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5061
    Illegal suffix found at 5105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5061
    Illegal SpeI site found at 5106
    Illegal PstI site found at 5120
    Illegal NotI site found at 5067
    Illegal NotI site found at 5113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5061
    Illegal BglII site found at 33
    Illegal BglII site found at 74
    Illegal BglII site found at 3682
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5061
    Illegal suffix found at 5106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5061
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5076
    Illegal SpeI site found at 5106
    Illegal PstI site found at 5120
    Illegal NgoMIV site found at 1895
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2693
    Illegal SapI.rc site found at 4194