Difference between revisions of "Part:BBa K4438102"

Latest revision as of 11:24, 12 October 2022

FASTmiR-222-D3

FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-222-D3, after transcription, binds to miR-222, the transcription product of BBa_K4438150. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.

Usage and Biology

Usage and Biology This part can be used for the detection of miR-222, which proves to be a biomarker for many diseases. The OFF(A) and ON(B) structure for the sensor was generated as given:

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 48
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 48
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 48
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 48
1000
COMPATIBLE WITH RFC[1000]

References

Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
 Usage and Biology
-This part can be used for the detection of miR-222, which proves to be a biomarker for many diseases. The ON and OFF structure for the sensor was generated as given:
+This part can be used for the detection of miR-222, which proves to be a biomarker for many diseases. The OFF(A) and ON(B) structure for the sensor was generated as given:
-[[File:https://static.igem.wiki/teams/4438/wiki/model/aptamer-design-collage/mirna-222-3.png|centre]]
+[[File:T--IISER-Tirupati_India--miRNA_222_3.png]]