Difference between revisions of "Part:BBa K4119010"

Latest revision as of 10:55, 12 October 2022

Plac->ΔperR->self-target->Cpa fdx terminator

it's Plac->ΔperR->self-target->Cpa fdx terminator

Results:

The results show that the target plasmid colonies PCR results appear band to eliminate the plasmid bacteria no band, at the same time, eliminate the target plasmid bacteria cannot survive in the medium containing resistance, prove that the target bacteria has completed plasmid elimination.

We hope to induce the elimination of plasmids by tetracycline after lactose-induced knockout of the gene of interest, and at present, we have only achieved lactose-induced knockout of the gene of interest with success. In addition, we designed plasmids solely to eliminate plasmids to verify whether the method of eliminating plasmids using tetr is feasible in Clostridium butyrate. We introduced a plasmid carrying the tetR gene in Clostridium butyrate and successfully eliminated it by tetracycline induction, demonstrating that this method is feasible. The work of knocking out the gene of interest and then eliminating the plasmid for time reasons is not completed.