Difference between revisions of "Part:BBa K4501002"

 
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Encodes for an shRNA against the 1741-1761 positions of the mRNA principal transcript of human MYC (HGNC:7553). It is composed of a sense and an anti-sense sequence separated by a loop domain. To optimize and stabilize the transcript, the C/D-box sequence is allocated at the position of the loop, substituting the loop.
 
Encodes for an shRNA against the 1741-1761 positions of the mRNA principal transcript of human MYC (HGNC:7553). It is composed of a sense and an anti-sense sequence separated by a loop domain. To optimize and stabilize the transcript, the C/D-box sequence is allocated at the position of the loop, substituting the loop.
[[BBa K4501002.jpeg]]
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[[File:BBa K4501002.jpeg]]
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Figure 1. Structure and stability of the shRNA
  
 
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Latest revision as of 10:43, 12 October 2022


anti-myc shRNA (1741-1761) in-loop C/D-box

Encodes for an shRNA against the 1741-1761 positions of the mRNA principal transcript of human MYC (HGNC:7553). It is composed of a sense and an anti-sense sequence separated by a loop domain. To optimize and stabilize the transcript, the C/D-box sequence is allocated at the position of the loop, substituting the loop.

BBa K4501002.jpeg Figure 1. Structure and stability of the shRNA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]