Difference between revisions of "Part:BBa K4488005"
 
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<partinfo>BBa_K4488005 short</partinfo>
 
<partinfo>BBa_K4488005 short</partinfo>
  
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Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102
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Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102
  
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<b>Usage:</b>
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<b>Usage</b> Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488027
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This part has been used in a composite part with Neae-Intimin for expression in TOP10 E.coli. See part BBa_K4488027.
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[[File:modelling-sf-h.gif|centre|thumb|700px| modelling of Nanobody h (3OGOH) to sfGFP]]
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[[File:modelling-sf-h.gif|centre|thumb|700px| Modelling of binding of Nanobody H (3OGO-H) to sfGFP made using python package LightDock]]
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References: Twair, A., Al-Okla, S., Zarkawi, M., & Abbady, A. Q. (2014). Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Molecular biology reports, 41(10), 6887–6898. https://doi.org/10.1007/s11033-014-3575-x
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:11, 12 October 2022


Anti-GFP Nanobody/sdAb

Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102

Usage Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488027

Modelling of binding of Nanobody H (3OGO-H) to sfGFP made using python package LightDock

References: Twair, A., Al-Okla, S., Zarkawi, M., & Abbady, A. Q. (2014). Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Molecular biology reports, 41(10), 6887–6898. https://doi.org/10.1007/s11033-014-3575-x

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]