Difference between revisions of "Part:BBa K283022:Design"

 
(→‎Source)
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K283022 short</partinfo>
 
<partinfo>BBa_K283022 short</partinfo>
 +
 +
The BBa_K283016 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the ''cheZ'' encoding gene was inserted between XbaI and SpeI.
  
 
<partinfo>BBa_K283022 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K283022 SequenceAndFeatures</partinfo>
Line 7: Line 8:
  
 
===Design Notes===
 
===Design Notes===
−
BBa_K283022
 
−
 
−
 
  
 
===Source===
 
===Source===
−
 
+
This part is composited from BBa_R0010, BBa_B0034 and BBa_K094100
−
The chez coding region is self-designed
+
−
The rbs and the pLac promomter are from the registry
+
  
 
===References===
 
===References===

Latest revision as of 17:05, 21 October 2009

pLacI-rbs-cheZ

The BBa_K283016 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the cheZ encoding gene was inserted between XbaI and SpeI.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 872
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This part is composited from BBa_R0010, BBa_B0034 and BBa_K094100

References