Difference between revisions of "Part:BBa K283017:Design"

 
 
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<partinfo>BBa_K283017 short</partinfo>
 
<partinfo>BBa_K283017 short</partinfo>
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This part was used to generate T7 RNA polymerase which can activate phage T7 promoter.
  
 
<partinfo>BBa_K283017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K283017 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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BBa_K283017
 
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===Source===
 
===Source===
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This part is composited from BBa_K283038, BBa_B0034 and BBa_K283047
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The T7polymerase, prosseA promoter is self-desgined
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The rbs is from the registry
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===References===
 
===References===

Latest revision as of 14:35, 21 October 2009

prosseA-rbs-T7 RNAP (Backbone: pSB1A2)

This part was used to generate T7 RNA polymerase which can activate phage T7 promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 372
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 114
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2219


Design Notes

Source

This part is composited from BBa_K283038, BBa_B0034 and BBa_K283047

References