Difference between revisions of "Part:BBa K283016"

Latest revision as of 14:30, 21 October 2009

pLacI-rbs-cheZ_histag-terminators

The pLac can be repressed by LacI protein which can be inhibited by IPTG. The CheZ protein is responsible for the dephosphorylation of CheY protein in bacteria flagella movement and is tagged by 6-Histidine tail. At the beginnning, we intend to use this part to control the speed of our Bactormotor by adjusting the concentration of inducer IPTG. However, we found the leaky expression is too high. Then our group combined it with to construct a new biobrick BBa_K283014. Unfortunately, we found that it still high. In the end, we created speed control device BBa_K283002 instead of it.This part does not pose any biological threat.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 872
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K283016 short</partinfo>
-The pLac promoter can be repressed by LacI protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by 6-Histidine tail.
+The pLac can be repressed by LacI protein which can be inhibited by IPTG. The CheZ protein is responsible for the dephosphorylation of CheY protein in bacteria flagella movement and is tagged by 6-Histidine tail. At the beginnning, we intend to use this part to control the speed of our Bactormotor by adjusting the concentration of inducer IPTG. However, we found the leaky expression is too high. Then our group combined it with to construct a new biobrick BBa_K283014. Unfortunately, we found that it still high. In the end, we created speed control device BBa_K283002 instead of it.This part does not pose any biological threat.
 <!-- Add more about the biology of this part here