Difference between revisions of "Part:BBa K283049:Design"

 
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<partinfo>BBa_K283049 short</partinfo>
 
<partinfo>BBa_K283049 short</partinfo>
  
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The BBa_K283002 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the tetR gene with LVA tag sequence was inserted between XbaI and SpeI.
 
<partinfo>BBa_K283049 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K283049 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
BBa_K283049
 
 
 
  
 
===Source===
 
===Source===
 
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This part is composited from BBa_R0010, BBa_B0034 and BBa_C0040
The panas promoter is self-designed
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The rbs, GFP and the terminator are from the registry
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===References===
 
===References===

Latest revision as of 17:20, 21 October 2009

pLacI-rbs-tetR(LVA)

The BBa_K283002 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the tetR gene with LVA tag sequence was inserted between XbaI and SpeI.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This part is composited from BBa_R0010, BBa_B0034 and BBa_C0040

References