Difference between revisions of "Part:BBa K4438160"
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<partinfo>BBa_K4438160 short</partinfo> | <partinfo>BBa_K4438160 short</partinfo> | ||
− | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for <partinfo>BBa_K4438100</partinfo> with <partinfo>BBa_J64997</partinfo> upstream. FASTmiR-222-D1_p, after transcription, binds to miR-222, the transcription product of <partinfo> | + | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for <partinfo>BBa_K4438100</partinfo> with <partinfo>BBa_J64997</partinfo> upstream. FASTmiR-222-D1_p, after transcription, binds to miR-222, the transcription product of <partinfo>BBa_K4438150</partinfo>. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | This part can be used for the detection of miR-222, which proves to be a biomarker for many diseases. The promoter sequence, in the beginning, enhances its usage in both in-vivo and in-vitro conditions. The | + | This part can be used for the detection of miR-222, which proves to be a biomarker for many diseases. The promoter sequence, in the beginning, enhances its usage in both in-vivo and in-vitro conditions. The ONFF(A) and ON(B) structure for the sensor was generated as given: |
+ | |||
+ | [[File:T--IISER-Tirupati_India--miRNA_222_1.png]] | ||
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Latest revision as of 08:05, 12 October 2022
FASTmiR-222-D1_p
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for BBa_K4438100 with BBa_J64997 upstream. FASTmiR-222-D1_p, after transcription, binds to miR-222, the transcription product of BBa_K4438150. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
Usage and Biology
This part can be used for the detection of miR-222, which proves to be a biomarker for many diseases. The promoter sequence, in the beginning, enhances its usage in both in-vivo and in-vitro conditions. The ONFF(A) and ON(B) structure for the sensor was generated as given:
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 70
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 70
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 70
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 70
- 1000COMPATIBLE WITH RFC[1000]
Notes
The split position of the complementary sequence in the sensor is ACCCAGTAGCCA….GAGTGAGCT
References
Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130