Difference between revisions of "Part:BBa K4477002:Design"

(→‎Design Notes)
(→‎Design Notes)
 
(2 intermediate revisions by the same user not shown)
Line 9: Line 9:
 
Amino acid sequence from IMGT was reverse transcribed and codon optimized for expression in <em> E. coli B </em> (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.
 
Amino acid sequence from IMGT was reverse transcribed and codon optimized for expression in <em> E. coli B </em> (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.
  
−
The variable heavy and constant 1 domains of this heavy chain are human in origin, while the constant 2 and 3 domains are mouse in origin. See Team Virginia 2022's Benchling folder <a href="https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/">here</a> for detailed annotations.
+
The variable heavy and constant 1 domains of this heavy chain are human in origin, while the constant 2 and 3 domains are mouse in origin. For detailed annotations, see Team Virginia 2022's Benchling folder here: https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/
  
 
===Source===
 
===Source===

Latest revision as of 08:00, 12 October 2022


Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG antibody - heavy chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 73
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1158
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence from IMGT was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.

The variable heavy and constant 1 domains of this heavy chain are human in origin, while the constant 2 and 3 domains are mouse in origin. For detailed annotations, see Team Virginia 2022's Benchling folder here: https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/

Source

Orticumab heavy chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635

References