Difference between revisions of "Part:BBa K4477001:Design"

(→‎Source)
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Amino acid sequence was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle).
+
Amino acid sequence acquired from database was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.
−
 
+
  
 +
Note that while the heavy chain of this antibody contains human and mouse sequences, this light chain is entirely composed of human DNA.
  
 
===Source===
 
===Source===

Latest revision as of 07:25, 12 October 2022


Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG antibody - light chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 382


Design Notes

Amino acid sequence acquired from database was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.

Note that while the heavy chain of this antibody contains human and mouse sequences, this light chain is entirely composed of human DNA.

Source

Anti-apoB(MDA) light chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635

References