Difference between revisions of "Part:BBa K4229064"

Latest revision as of 12:02, 12 October 2022

mTurquoise2 with C-Terminal SnoopCatcher

mTurquoise (together with mVenus2,[BBa_K4229064]) was used as a reporter by fusing it with a SpyCatcher, enabling recruitment by the SpyTag. We used this fusion protein to test the functionality of our compartments: the minimal (BBa_K4229047) and full wiffleball (BBa_K4229049), and SPD5 (BBa_K4229078). All compartmentalisation proteins have an N-terminal SpyTag. For experimental data please refer to the data listed in Biobrick BBa_K4229067.

Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 763
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 763
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 763
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 763
Illegal AgeI site found at 28
Illegal AgeI site found at 924
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229064 short</partinfo>
-mTurqouise2 is with mVenus2(BBa_K4229062) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the snoopCatcher fused on the C-terminal of the protein to make it recruitable inside those compartments.
+mTurquoise (together with mVenus2,[BBa_K4229064]) was used as a reporter by fusing it with a SpyCatcher, enabling recruitment by the SpyTag. We used this fusion protein to test the functionality of our compartments: the minimal (BBa_K4229047) and full wiffleball (BBa_K4229049), and SPD5 (BBa_K4229078). All compartmentalisation proteins have an N-terminal SpyTag. For experimental data please refer to the data listed in Biobrick BBa_K4229067.
 [[File:ReporterCatched.jpg|800px|thumb|left| Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball]]