Difference between revisions of "Part:BBa K4438161:Experience"
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===Applications of BBa_K4438161=== | ===Applications of BBa_K4438161=== | ||
+ | [[File:T--IISER-Tirupati_India--Fluroscence_miR-222_(30_min).png]] | ||
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+ | [[File:T--IISER-Tirupati_India--Fluroscence_miR_222_(1_hr).png]] | ||
+ | Depicting fluorescence readout of FASTmiR-222-D2 after 30 mins and 1 hour incubation respectively.Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA | ||
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+ | Explanation: After 30mins of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-222 sensor was increased in concentration. An average fold-change of 2.69 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 6.048 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM. | ||
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+ | After 1 hour, an average fold-change of 3.07 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 7.423 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM. | ||
===User Reviews=== | ===User Reviews=== |
Latest revision as of 02:58, 14 October 2022
Applications of BBa_K4438161
Depicting fluorescence readout of FASTmiR-222-D2 after 30 mins and 1 hour incubation respectively.Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA
Explanation: After 30mins of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-222 sensor was increased in concentration. An average fold-change of 2.69 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 6.048 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.
After 1 hour, an average fold-change of 3.07 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 7.423 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.
User Reviews
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