Difference between revisions of "Part:BBa K4444010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | |||
| + | <u>Modifications to RELEASE: | ||
| + | |||
| + | </u>- Furin cut site is replaced with PhoCl | ||
| + | |||
| + | - Transmembrane domains, TEV cut site and ER retention motif are removed | ||
| + | |||
| + | - GFP is replaced with mRuby | ||
| + | |||
| + | <u>Components: | ||
| + | |||
| + | </u> mRuby, PhoCl, and transmembrane domain | ||
===Source=== | ===Source=== | ||
| − | + | ||
| + | RELEASE: https://www.addgene.org/179642/ | ||
| + | |||
| + | PhoCl: https://www.addgene.org/164037/ | ||
| + | |||
| + | mRuby: Lab member's plasmids (David Gonzalez-Martinez) | ||
===References=== | ===References=== | ||
Latest revision as of 08:10, 12 October 2022
PhoCl Shed mRuby
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 52
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 769
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1654
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1288
Illegal SapI.rc site found at 73
Design Notes
Modifications to RELEASE:
- Furin cut site is replaced with PhoCl
- Transmembrane domains, TEV cut site and ER retention motif are removed
- GFP is replaced with mRuby
Components:
mRuby, PhoCl, and transmembrane domain
Source
RELEASE: https://www.addgene.org/179642/
PhoCl: https://www.addgene.org/164037/
mRuby: Lab member's plasmids (David Gonzalez-Martinez)
References
Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.
Zhang, W., Lohman, A., Zhuravlova, Y. et al. Optogenetic control with a photocleavable protein, PhoCl. Nat Methods 14, 391–394 (2017). https://doi.org/10.1038/nmeth.4222