Difference between revisions of "Part:BBa K4444011:Design"

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===Design Notes===
 
===Design Notes===
−
n/a
+
<u>Modifications to RELEASE:
 +
 
 +
</u> - TEV cut site is replaced with PhoCl
 +
 
 +
- Furin cut site is removed
 +
 
 +
- GFP is replaced with mRuby
 +
 
 +
<u>Components:
 +
 
 +
</u>mRuby, tri-transmembrane domain, Phocl , and ER retention motif
  
  
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===Source===
 
===Source===
  
−
Addgene: https://www.addgene.org/179642/
+
RELEASE: https://www.addgene.org/179642/
 +
 
 +
PhoCl: https://www.addgene.org/164037/
 +
 
 +
mRuby: Lab member's plasmids (David Gonzalez-Martinez)
  
 
===References===
 
===References===

Latest revision as of 08:11, 12 October 2022


PhoCl Display mRuby


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 895
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1417
    Illegal SapI.rc site found at 73


Design Notes

Modifications to RELEASE:

- TEV cut site is replaced with PhoCl

- Furin cut site is removed

- GFP is replaced with mRuby

Components:

mRuby, tri-transmembrane domain, Phocl , and ER retention motif


Source

RELEASE: https://www.addgene.org/179642/

PhoCl: https://www.addgene.org/164037/

mRuby: Lab member's plasmids (David Gonzalez-Martinez)

References

Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.

Zhang, W., Lohman, A., Zhuravlova, Y. et al. Optogenetic control with a photocleavable protein, PhoCl. Nat Methods 14, 391–394 (2017). https://doi.org/10.1038/nmeth.4222