Difference between revisions of "Part:BBa K4241802"
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<partinfo>BBa_K4241802 short</partinfo> | <partinfo>BBa_K4241802 short</partinfo> | ||
+ | <partinfo>BBa_K4241802 SequenceAndFeatures</partinfo> | ||
+ | ===Overview=== | ||
+ | This composite part is an inducible high-expression system to produce Cecropin B, an antimicrobial peptide in microalgae. The expression of this part is triggered by nitrogen starvation. This part is based upon research from the following publication: https://www.nature.com/articles/s41598-020-69620-9 | ||
+ | |||
+ | ===Usage and Biology=== | ||
This part is used for the inducible expression of Cecropin B in a high-expression manner in microalgae. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression. | This part is used for the inducible expression of Cecropin B in a high-expression manner in microalgae. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression. | ||
+ | |||
+ | ===Parts that comprise this composite component=== | ||
+ | Nitrogen Starvation Inducible promoter 1 (Microalgae) - Part:BBa_K4241790 <br/> | ||
+ | CvNDI_UTR - BBa_K4241799 <br/> | ||
+ | Cecropin B Q53 - Part:BBa_K4241793 <br/> | ||
+ | TRAmy3D_UTR - BBa_K4241800 <br/> | ||
+ | TRAmy3D - BBa_K4241794 <br/> | ||
+ | |||
+ | ===References=== | ||
+ | Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9 | ||
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Latest revision as of 02:14, 12 October 2022
CvNDI1_CeB_TRAmy3D
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1506
Illegal PstI site found at 55
Illegal PstI site found at 404 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1506
Illegal PstI site found at 55
Illegal PstI site found at 404 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1506
Illegal PstI site found at 55
Illegal PstI site found at 404 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1506
Illegal PstI site found at 55
Illegal PstI site found at 404
Illegal NgoMIV site found at 264
Illegal NgoMIV site found at 1086 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 849
Overview
This composite part is an inducible high-expression system to produce Cecropin B, an antimicrobial peptide in microalgae. The expression of this part is triggered by nitrogen starvation. This part is based upon research from the following publication: https://www.nature.com/articles/s41598-020-69620-9
Usage and Biology
This part is used for the inducible expression of Cecropin B in a high-expression manner in microalgae. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.
Parts that comprise this composite component
Nitrogen Starvation Inducible promoter 1 (Microalgae) - Part:BBa_K4241790
CvNDI_UTR - BBa_K4241799
Cecropin B Q53 - Part:BBa_K4241793
TRAmy3D_UTR - BBa_K4241800
TRAmy3D - BBa_K4241794
References
Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9