Difference between revisions of "Part:BBa K4241801"
 
Line 5: Line 5:
  
 
===Overview===
 
===Overview===
−
This composite part is an inducible high-expression system to produce Ulp1 for SUMO-cleavage in microalgae. The expression of this part is triggered by nitrogen starvation.
+
This composite part is an inducible high-expression system to produce Ulp1 for SUMO-cleavage in microalgae. The expression of this part is triggered by nitrogen starvation. This part is based upon research from the following publication: https://www.nature.com/articles/s41598-020-69620-9
  
 
===Usage and Biology===
 
===Usage and Biology===
Line 16: Line 16:
 
TRAmy3D_UTR - BBa_K4241800 <br/>
 
TRAmy3D_UTR - BBa_K4241800 <br/>
 
TRAmy3D - BBa_K4241794 <br/>
 
TRAmy3D - BBa_K4241794 <br/>
 +
 +
===References===
 +
Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., &amp; Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9
  
  

Latest revision as of 02:15, 12 October 2022


CvNDI1_Ulp1_TRAmy3D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2058
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal PstI site found at 1242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2058
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal PstI site found at 1242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1376
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2058
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal PstI site found at 1242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2058
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal PstI site found at 1242
    Illegal NgoMIV site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849

Overview

This composite part is an inducible high-expression system to produce Ulp1 for SUMO-cleavage in microalgae. The expression of this part is triggered by nitrogen starvation. This part is based upon research from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Usage and Biology

This part is used for the inducible expression of Ulp1 in a high-expression manner. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.

Parts that comprise this composite component

Nitrogen Starvation Inducible promoter 1 (Microalgae) - Part:BBa_K4241790
CvNDI_UTR - BBa_K4241799
Ulp1_Active Domain (Microalgae) - BBa_K4241797
TRAmy3D_UTR - BBa_K4241800
TRAmy3D - BBa_K4241794

References

Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9