Difference between revisions of "Part:BBa K4438600"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This aptamer has a high affinity for testosterone with KD being 1.8 ± 0.3 nM. This was calculated using Apta-PCR affinity assay (APAA). This part can be used to develop aptasensors that can detect different concentrations of testosterone in biological samples [1]. | This aptamer has a high affinity for testosterone with KD being 1.8 ± 0.3 nM. This was calculated using Apta-PCR affinity assay (APAA). This part can be used to develop aptasensors that can detect different concentrations of testosterone in biological samples [1]. | ||
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Latest revision as of 18:26, 13 October 2022
Aptamer_T6
Aptamer_T6 is a single-stranded DNA aptamer having 86 nucleotides. Figure 1A) Shows the secondary structure of the aptamer and its minimum free energy.
Usage and Biology
This aptamer has a high affinity for testosterone with KD being 1.8 ± 0.3 nM. This was calculated using Apta-PCR affinity assay (APAA). This part can be used to develop aptasensors that can detect different concentrations of testosterone in biological samples [1].
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 31
Illegal SpeI site found at 67 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 31
Illegal SpeI site found at 67 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 31
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 31
Illegal SpeI site found at 67 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 31
Illegal SpeI site found at 67 - 1000COMPATIBLE WITH RFC[1000]
References
Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.