Difference between revisions of "Part:BBa K4438600"

 
 
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__NOTOC__
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<partinfo>BBa_K4438600 short</partinfo>
  
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Aptamer_T6 is a single-stranded DNA aptamer having 86 nucleotides. Figure 1A) Shows the secondary structure of the aptamer and its minimum free energy.
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===Usage and Biology===
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This aptamer has a high affinity for testosterone with KD being 1.8 ± 0.3 nM. This was calculated using Apta-PCR affinity assay (APAA). This part can be used to develop aptasensors that can detect different concentrations of testosterone in biological samples [1].
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[[File:T--IISER-Tirupati_India--T6_2.png]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4438600 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4438600 parameters</partinfo>
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===References===
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Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.

Latest revision as of 18:26, 13 October 2022

Aptamer_T6

Aptamer_T6 is a single-stranded DNA aptamer having 86 nucleotides. Figure 1A) Shows the secondary structure of the aptamer and its minimum free energy.

Usage and Biology

This aptamer has a high affinity for testosterone with KD being 1.8 ± 0.3 nM. This was calculated using Apta-PCR affinity assay (APAA). This part can be used to develop aptasensors that can detect different concentrations of testosterone in biological samples [1]. T--IISER-Tirupati India--T6 2.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 31
    Illegal SpeI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
    Illegal SpeI site found at 67
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 31
    Illegal SpeI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 31
    Illegal SpeI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.