Difference between revisions of "Part:BBa K4438605"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Few bases of this part are complementary (Watson-Crick base pairing) to T6 (BBa_K4438600) and are designed to block its sequence motif. Motif, mostly associated with the loops, is linked to the formation of the specific binding site for testosterone [1]. Figure 2D) Shows the secondary structure of both parts hybridised at 37° Celsius. The hormone binds with T6 (BBa_K4438600) with high affinity and displaces the T6_Trigger_3_phi29. | + | Few bases of this part are complementary (Watson-Crick base pairing) to T6 (<partsinfo>BBa_K4438600</partsinfo>) and are designed to block its sequence motif. Motif, mostly associated with the loops, is linked to the formation of the specific binding site for testosterone [1]. Figure 2D) Shows the secondary structure of both parts hybridised at 37° Celsius. The hormone binds with T6 (<partsinfo>BBa_K4438600</partsinfo>) with high affinity and displaces the T6_Trigger_3_phi29. |
This part has complete complementarity with part T6_Binder_3(BBa_K4438602). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. | This part has complete complementarity with part T6_Binder_3(BBa_K4438602). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. | ||
Different levels of testosterone, an anabolic androgenic steroid (AAS), in biological fluids can be detected using all these three parts. | Different levels of testosterone, an anabolic androgenic steroid (AAS), in biological fluids can be detected using all these three parts. | ||
| − | + | [[File:T--IISER-Tirupati_India--T6_3.png]] | |
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Latest revision as of 12:45, 12 October 2022
T6_Trigger_3_w/oPol
T6_Trigger_3_w/oPol is single-stranded DNA having 29 nucleotides. Figure 1B)Illustrates the secondary structure and its minimum free energy. The 3’ end has ssDNA sense T7 promoter sequence.
Usage and Biology
Few bases of this part are complementary (Watson-Crick base pairing) to T6 (<partsinfo>BBa_K4438600</partsinfo>) and are designed to block its sequence motif. Motif, mostly associated with the loops, is linked to the formation of the specific binding site for testosterone [1]. Figure 2D) Shows the secondary structure of both parts hybridised at 37° Celsius. The hormone binds with T6 (<partsinfo>BBa_K4438600</partsinfo>) with high affinity and displaces the T6_Trigger_3_phi29.
This part has complete complementarity with part T6_Binder_3(BBa_K4438602). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
Different levels of testosterone, an anabolic androgenic steroid (AAS), in biological fluids can be detected using all these three parts.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 4
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 4
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 4
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 4
- 1000COMPATIBLE WITH RFC[1000]
References
Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.