Difference between revisions of "Part:BBa K4438601"

 
 
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<partinfo>BBa_K4438601 short</partinfo>
  
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T6_Trigger_1_midtrigger_phi29 is single-stranded DNA having 27 nucleotides. Figure 1B) Illustrates the secondary structure and its minimum free energy.
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===Usage and Biology===
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Few bases of this part are complementary (Watson-Crick base pairing) to T6 <partinfo>BBa_K4438600</partinfo> and are  designed to block its sequence motif. Motif, mostly associated with the loops, is linked to the formation of the specific binding site for testosterone [1]. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. The hormone binds with T6 <partinfo>BBa_K4438600</partinfo> with high affinity and displaces the T6_Trigger_1_midtrigger_phi29.
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This part has complete complementarity with the part T6_Binder_1(<partinfo>BBa_K4438602</partinfo>). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
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Different levels of testosterone, an anabolic androgenic steroid (AAS), in biological fluids can be detected using all these three parts.
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[[File:T--IISER-Tirupati_India--T6_1.png]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4438601 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4438601 parameters</partinfo>
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===References===
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Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.

Latest revision as of 18:29, 13 October 2022

T6_Trigger_1_midtrigger_phi29


T6_Trigger_1_midtrigger_phi29 is single-stranded DNA having 27 nucleotides. Figure 1B) Illustrates the secondary structure and its minimum free energy.


Usage and Biology

Few bases of this part are complementary (Watson-Crick base pairing) to T6 BBa_K4438600 and are designed to block its sequence motif. Motif, mostly associated with the loops, is linked to the formation of the specific binding site for testosterone [1]. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. The hormone binds with T6 BBa_K4438600 with high affinity and displaces the T6_Trigger_1_midtrigger_phi29. This part has complete complementarity with the part T6_Binder_1(BBa_K4438602). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of testosterone, an anabolic androgenic steroid (AAS), in biological fluids can be detected using all these three parts. T--IISER-Tirupati India--T6 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.