Difference between revisions of "Part:BBa K4438131"
(One intermediate revision by one other user not shown) | |||
Line 1: | Line 1: | ||
+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4438131 short</partinfo> | ||
+ | |||
+ | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for <partinfo>BBa_K4438111</partinfo> with <partinfo>BBa_J64997</partinfo> upstream and <partinfo>BBa_K731721</partinfo> downstream along with some spacer sequences <partinfo>BBa_K3831027</partinfo> (at 5’ end) and <partinfo>BBa_K4438136</partinfo> (at 3’ end). FASTmiR-30c-D2_s, after transcription, binds to miR-30c, the transcription product of <partinfo>BBa_K4438153</partinfo>. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence. | ||
+ | |||
+ | |||
+ | ===Usage and Biology=== | ||
+ | This part can be used for the detection of miR-30c, which proves to be a biomarker for many diseases. A promoter sequence added in the beginning enhances its usage in both in-vivo and in-vitro conditions. The OFF(A) and ON(B) structure for the sensor was generated as given: | ||
+ | [[File:T--IISER-Tirupati_India--miRNA_30c1_2.png]] | ||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4438131 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4438131 parameters</partinfo> | ||
+ | <!-- --> | ||
+ | ===Notes=== | ||
+ | The split position of the complementary sequence in the sensor is GCTGAGAGTGTAGG….ATGTTTACA | ||
+ | |||
+ | ===References=== | ||
+ | Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130 |
Latest revision as of 10:14, 12 October 2022
FASTmiR-30c-D2_s
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for BBa_K4438111 with BBa_J64997 upstream and BBa_K731721 downstream along with some spacer sequences BBa_K3831027 (at 5’ end) and BBa_K4438136 (at 3’ end). FASTmiR-30c-D2_s, after transcription, binds to miR-30c, the transcription product of BBa_K4438153. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
Usage and Biology
This part can be used for the detection of miR-30c, which proves to be a biomarker for many diseases. A promoter sequence added in the beginning enhances its usage in both in-vivo and in-vitro conditions. The OFF(A) and ON(B) structure for the sensor was generated as given:
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 87
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 87
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 87
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 87
- 1000COMPATIBLE WITH RFC[1000]
Notes
The split position of the complementary sequence in the sensor is GCTGAGAGTGTAGG….ATGTTTACA
References
Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130