Difference between revisions of "Part:BBa K4060021"

Latest revision as of 01:34, 12 October 2022

pelB leader sequence with aprN (without native signal peptide sequence)

aprN (without natural singal peptide) + pelB. pelB leader sequence which is a gene fragment of pelB can help to direct to E. coli periplasmic membrane. The aprN gene is the gene of Nattokinase. Nattokinase (subtilisin NAT) is a kind of extracellular alkaline serine protease, which has the ability to prevent deep vein thrombosis (DVT) due to its fibrinolytic activity.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 67
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 67
Illegal NotI site found at 211
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 67
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 67
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 345
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 349

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4060021 short</partinfo>
-The expression of Nattokinase in Escherichia coli is prone to the formation of inclusion bodies, so we use weak promoter and RBS combination to express Nattokinase. In order to have enough time and space to fold and secrete nattokinase into the intestine.
+aprN (without natural singal peptide) + pelB.
+pelB leader sequence which is a gene fragment of pelB can help to direct to E. coli periplasmic membrane. The aprN gene is the gene of Nattokinase. Nattokinase (subtilisin NAT) is a kind of extracellular alkaline serine protease, which has the ability to prevent deep vein thrombosis (DVT) due to its fibrinolytic activity.
 <!-- Add more about the biology of this part here