Difference between revisions of "Part:BBa K4438108:Experience"
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===Applications of BBa_K4438108=== | ===Applications of BBa_K4438108=== | ||
+ | [[File:T--IISER-Tirupati_India--fluroscence_miR-146_(30_min).png]] | ||
+ | [[File:T--IISER-Tirupati_India--fluroscence_miR-146-(1_hr).png]] | ||
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+ | Depicting the fluorescence readout of FASTmiR-146a-D4 (design 4) after 30 mins and 1 hour incubation respectively. Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA | ||
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+ | Explanation: After 30 minutes of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-146a sensor was increased in concentration. An average fold-change of 1.327 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.319 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM. | ||
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+ | While after 1 hour of incubation with 5X sensor buffer and dye, an average fold-change of 1.41 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.311 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM. | ||
===User Reviews=== | ===User Reviews=== | ||
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Latest revision as of 03:06, 14 October 2022
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Applications of BBa_K4438108
Depicting the fluorescence readout of FASTmiR-146a-D4 (design 4) after 30 mins and 1 hour incubation respectively. Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA
Explanation: After 30 minutes of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-146a sensor was increased in concentration. An average fold-change of 1.327 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.319 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.
While after 1 hour of incubation with 5X sensor buffer and dye, an average fold-change of 1.41 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.311 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.
User Reviews
UNIQ291dc0f3a49c75be-partinfo-00000000-QINU UNIQ291dc0f3a49c75be-partinfo-00000001-QINU