Difference between revisions of "Part:BBa K245046"

 
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{{Curation/ccdb}}
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All of RFC 37 vectors (BB-NIC-II-HisN - BBa_K245005, BB-NIC-II-NC - BBa_K245006, BB-NIC-II-HisC - BBa_K245007 and BB-NIC-III-HisN - BBa_K245008) have a ccdB gene inserted in their multiple cloning site. CcdB serves as positive selection marker since it is lethal to almost all BioBrick cell strains (E.coli DB3.1 strain is an exeption). While inserting BioBrick parts in plasmid, there exist a possibility of contamination of ligation mixture with uncut vector. If vector in which one plans to insert BioBrick part contains ccdB domain in multiple cloning site as the ones listed above, cells that were transformed with unmodified vector express ccdB protein which results in cell death. For this reason any vector containing ccdB could only be propagated in resistant cell strains like DB3.1. This part comprises only active ccdB part without inactive ccdA.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:26, 9 July 2015

ccdB


All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ


All of RFC 37 vectors (BB-NIC-II-HisN - BBa_K245005, BB-NIC-II-NC - BBa_K245006, BB-NIC-II-HisC - BBa_K245007 and BB-NIC-III-HisN - BBa_K245008) have a ccdB gene inserted in their multiple cloning site. CcdB serves as positive selection marker since it is lethal to almost all BioBrick cell strains (E.coli DB3.1 strain is an exeption). While inserting BioBrick parts in plasmid, there exist a possibility of contamination of ligation mixture with uncut vector. If vector in which one plans to insert BioBrick part contains ccdB domain in multiple cloning site as the ones listed above, cells that were transformed with unmodified vector express ccdB protein which results in cell death. For this reason any vector containing ccdB could only be propagated in resistant cell strains like DB3.1. This part comprises only active ccdB part without inactive ccdA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216