Difference between revisions of "Part:BBa K4451011"
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BBa_K4451008 encodes the gp104 protein from the 77 phage. This protein has been reported to interact with DnaI (helicase loader) and shuts down host replication (Liu et al., 2004). | BBa_K4451008 encodes the gp104 protein from the 77 phage. This protein has been reported to interact with DnaI (helicase loader) and shuts down host replication (Liu et al., 2004). | ||
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| + | ===Description=== | ||
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| + | An inhibitor of DNA replication from the 77 phage, which causes growth rate suppression in Staphylococcus aureus. | ||
| + | The gp104 gene encodes a small inhibitory protein that targets and inhibits the DnaI protein in S. aureus, itself responsible for helicase loading onto replicating DNA and thus a crucial part of the replisome. Its inhibition shows potent growth rate suppression due to the stalling of DNA replication initiation. To date, no evidence has been shown to suggest its function in Escherichia coli, and it is worth noting that E. coli itself does not have a closely related DnaI homolog (Liu et al., 2004). | ||
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| + | https://static.igem.wiki/teams/4939/wiki/assets/assets/registry-assets/bba-k4451011-meanlog2od.png | ||
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| + | Figure 1: Mean log2(OD) against time for gp104, concentrations refer to the IPTG used to induce the pLac promoter regulating gp104 | ||
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| + | Figure 1 shows there was no significant change in growth between various concentrations of IPTG used to induce the pLac promoter in either gp104 or the control. This is likely because E. coli itself does not have a closely related DnaI homolog. | ||
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Latest revision as of 15:56, 12 October 2023
77 gp104
BBa_K4451008 encodes the gp104 protein from the 77 phage. This protein has been reported to interact with DnaI (helicase loader) and shuts down host replication (Liu et al., 2004).
Description
An inhibitor of DNA replication from the 77 phage, which causes growth rate suppression in Staphylococcus aureus. The gp104 gene encodes a small inhibitory protein that targets and inhibits the DnaI protein in S. aureus, itself responsible for helicase loading onto replicating DNA and thus a crucial part of the replisome. Its inhibition shows potent growth rate suppression due to the stalling of DNA replication initiation. To date, no evidence has been shown to suggest its function in Escherichia coli, and it is worth noting that E. coli itself does not have a closely related DnaI homolog (Liu et al., 2004).
Figure 1: Mean log2(OD) against time for gp104, concentrations refer to the IPTG used to induce the pLac promoter regulating gp104
Figure 1 shows there was no significant change in growth between various concentrations of IPTG used to induce the pLac promoter in either gp104 or the control. This is likely because E. coli itself does not have a closely related DnaI homolog.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]