Difference between revisions of "Part:BBa K4229003"
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The low affinity tryptophan permease TnaB is involved in the transport of tryptophan across the cytoplasmic membrane. It is a multi-pass membrane protein. | The low affinity tryptophan permease TnaB is involved in the transport of tryptophan across the cytoplasmic membrane. It is a multi-pass membrane protein. | ||
| − | As one part of our toolbox to produce indigo/ | + | As one part of our toolbox to produce indigo/indirubin, we express TnaB to improve the cellular uptake of L-tryptophan. |
[[File:Grafik indigo weier bg.png|900px|thumb|left| | [[File:Grafik indigo weier bg.png|900px|thumb|left| | ||
Latest revision as of 11:10, 12 October 2022
TnaB tryptophan transport protein
The low affinity tryptophan permease TnaB is involved in the transport of tryptophan across the cytoplasmic membrane. It is a multi-pass membrane protein.
As one part of our toolbox to produce indigo/indirubin, we express TnaB to improve the cellular uptake of L-tryptophan.
Figure 1: Schematic representation of the indigo/indirubin pathway. L-Tryptophan is imported by the membrane protein TnaB (low affinity tryptophan permease). L-tryptophan is cleaved into indole, NH4+ and pyruvate by the Tryptophanase TnaA. The reaction continues by the hydroxylation of indole through XiaI. To enhance the effectivity of this enzyme, the NAD(P)H-flavin reductase provides XiaI with FADH2 by adding Hydrogen to FAD. Finally, indole is transformed to either 3-Hydroxyindole or 2-Hydroxyindole. These two substances spontaneously react to 3-Oxindole and 2-Oxindole through the secession of hydrogen from the OH-group. Through spontaneous dimerization indigo and indirubin are formed. Graphic adapted from [1].
References:
[1] H. Yin et al., “Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli,” ACS Omega, vol. 6, no. 31, pp. 20569–20576, 2021, doi: 10.1021/acsomega.1c02679.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]